Notice of the Ministry of Agriculture on Printing and Distributing the Emergency Monitoring Plan for Animal H7N9 Avian Influenza and the Emergency Disposal Guide for Animal H7N9 Avian Influenza (Trial
To further strengthen animalsH7N9Avian influenza prevention and control work, comprehensively strengthen epidemic investigation and virus monitoring, and deal with emergencies in a timely manner. Our department organized and formulated the "Animals"H7N9Avian Influenza Emergency Monitoring Program and AnimalsH7N9Guidelines for Emergency Disposal of Avian Influenza (Trial) are hereby printed and distributed to you, please follow them.
The Ministry of Agriculture of the People’s Republic of China
2013yearfourmoonsevensun
animalH7N9Avian influenza emergency monitoring program
I. Purpose of monitoring
Grasp and understandH7N9The source, host range, transmission route and harm degree of avian influenza virus in animal groups; In order to clear the animal population in timeH7N9The pathogen of avian influenza provides scientific basis.
Second, the monitoring scope
(1) Core monitoring area
Human infection has occurred.H7N9Avian Influenza Cases and Animals Confirmed by National Avian Influenza Reference LaboratoryH7N9Avian influenza positive provinces.
(2) Key monitoring areas
Provinces adjacent to the core monitoring area.
(3) General monitoring area
Provinces outside the above two types of monitoring areas.
Third, the monitoring object
Chickens, waterfowl (ducks and geese) and artificially raised pigeons and quails; Wild birds; Live pigs.
Fourth, the number of monitoring
(1) Live poultry trading market
Each market collects not less thanthirtyThe corresponding blood samples, throat and cloacal swabs of each poultry should cover as many kinds of poultry and stalls as possible.
Each province in the core monitoring area collects all live poultry trading markets, and each county (city, district) in the key monitoring area collects at least.oneA live poultry trading market, other monitoring areas in each place (city, state) at least collection.oneA live poultry trading market.
(2) Poultry slaughterhouses
Each poultry slaughterhouse collectsthreeMore than 10 poultry groups, and each poultry group is collected.thirtyCorresponding blood samples, throat and cloacal swabs of 10 poultry.
All poultry slaughterhouses are collected in each province in the core monitoring area, and at least in each province in the key monitoring area.10A poultry slaughterhouse, other monitoring areas at least collected in each province.fiveA poultry slaughterhouse.
(3) Poultry farms (villages)
Each poultry farm (village) shall collect not less thanthirtyThe corresponding blood samples, throat and cloaca swabs of each poultry should cover as many poultry houses (households) as possible.
Each county (city, district) in the core monitoring area shall collect at leastthirtyPoultry farms (villages); Each province in the key monitoring area shall collect at leasttwentyPoultry farms (villages); Other monitoring areas shall be collected at least in each province.10A poultry farm (village).
Once the pathogen test results of samples collected by poultry farms (villages) are positive, all poultry farms (villages) in the county where the farm (village) is located shall be monitored.
(4) Habitat of wild birds
Collect fresh feces of wild birds. Collect throat and cloaca swabs from wild birds that can be captured.
(5) Pig slaughterhouse
Each slaughterhouse shall collect nasal swab of pigs at least.thirtyShare. Each province in the core monitoring area shall collect at leasttwentySlaughterhouse, the key monitoring area of each province at least collection.10Slaughterhouse, other monitoring areas at least collected in each province.fiveA slaughterhouse.
(6) Others
Each province shall determine the sampling quantity of environmental samples at the above-mentioned sites by itself.
V. Detection methods
(1) Serological detection
Using hemagglutination inhibition (HI) test, detection of serumH7Hemagglutinin antibody of subtype avian influenza virus. Refer to Diagnostic Techniques for Highly Pathogenic Avian Influenza (GB/T 18936–2003).HIThe experiment was carried out,HIAntibody level ≥2fourThe result was positive.
(2) Pathogenic detection
Recommended by the Ministry of AgricultureRT–PCROr fluorescenceRT–PCRDetection method: throat and cloaca swab samples are detected.H7Subtype avian influenza virusHAGene fragment. According to the recommended reagent (box) instructions.
VI. Monitoring Time
do all one can to do sth2013yearfourFinish before the end of the month.
VII. Division of tasks
The Veterinary Bureau of the Ministry of Agriculture is responsible for organizing the implementation, the China Animal Disease Prevention and Control Center is responsible for work coordination and data collection and analysis, and the National Avian Influenza Reference Laboratory is responsible for providing technical support and diagnostic reagent supply.
The provincial veterinary administrative department is responsible for organizing the implementation of the monitoring work in this area, the provincial animal disease prevention and control institutions are responsible for the pathogen detection, and the city and county animal disease prevention and control institutions are responsible for the serological detection, and may also entrust relevant units to carry out the detection.
VIII. Relevant requirements
(a) the implementation of the express system for positive results.Positive samples were detected by serology of animal disease prevention and control institutions at city and county levels, and sent to provincial animal disease prevention and control institutions for pathogen detection, with positive results.2Report to the competent veterinary department at the same level within hours. Provincial animal epidemic disease prevention and control institutionsoneReport the positive results to the provincial veterinary authorities and China Animal Disease Prevention and Control Center within hours.24Send the positive samples to the National Avian Influenza Reference Laboratory within hours. China animal epidemic disease prevention control centeroneReport the situation to the Veterinary Bureau of the Ministry of Agriculture within hours. The National Avian Influenza Reference Laboratory immediately reported the diagnosis to the Veterinary Bureau of the Ministry of Agriculture.
(two) the implementation of weekly reporting system for monitoring.Provincial animal disease prevention and control institutions every Monday.10The monitoring results will be summarized and reported to China Animal Disease Prevention and Control Center through the National Animal Disease Monitoring and Epidemic Information System. China Animal Disease Prevention and Control Center every Monday.twelveThe summary results will be reported to the Veterinary Bureau of the Ministry of Agriculture and copied to China Animal Health and Epidemiology Center.
(3) Carry out retrospective monitoring.China Animal Disease Prevention and Control Center, China Animal Health and Epidemiology Center and provincial animal disease prevention and control institutions2012yearoneRelevant samples preserved since June, carried out.H7Retrospective monitoring of subtype avian influenza.
(4) Make a good record of sample collection.Standardize the filling of sampling records to ensure that the records are true, accurate and traceable. All samples should be registered, reviewed, signed and sealed step by step.
(5) Standardize the disposal of positive cases.Monitoring found positive, in strict accordance with the "animals"H7N9Guidelines for Emergency Disposal of Avian Influenza (Trial).
Contact information:
veterinary bureau of the ministry of agriculture
Contact: Wei Wu, Tel:010–59191401, fax:010–59192861;
China animal epidemic disease prevention control center
Contact: Fu Wen, Tel:010–59194601, fax:010–59194711;
China Animal Health and Epidemiology Center
Contact: Shen Chaojian, Tel:0532–85648038, fax:0532–85653716;
National Avian Influenza Reference Laboratory
Contact: Deng Guohua, Tel:13946057836; Fax:0451–51997166.
animalH7N9Guidelines for Emergency Disposal of Avian Influenza (Trial)
I. Scope of application
This guide stipulates that animalsH7N9Positive confirmation, disposal, emergency epidemiological investigation and personnel protection of avian influenza.
Second, positive confirmation
H7Subtype reverse transcription–Polymerase chain reaction (RT–PCR) or fluorescent reverse transcription–Polymerase chain reaction (fluorescenceRT–PCR) test results are positive, forH7Subtype avian influenza virus infection is positive.
Provincial animal disease prevention and control institutions diagnosed asH7If the subtype avian influenza virus infection is suspected to be positive, it shall be sent to the National Avian Influenza Reference Laboratory to review the results. The National Avian Influenza Reference Laboratory will carry out a review and other related work before making a diagnosis. According to the final diagnosis results, the Ministry of Agriculture confirmed thatH7Subtype avian influenza virus infection is positive.
Third, positive treatment
Infected group refers to the animal group where the positive samples are collected, including the following three types: one is the same animal in the farm, the other is the same poultry in the live poultry market, and the third is the same poultry in the rural area.
Diagnosed by provincial animal disease prevention and control institutions as followsH7If subtype avian influenza virus is suspected to be positive, all animals in the field (village) where the infected group is located shall be restricted from moving.
Confirmed by the Ministry of Agriculture asH7If the subtype avian influenza virus is positive, all animals in the infected group shall be culled, the culled animals and their products shall be treated harmlessly, strict disinfection measures shall be implemented for the internal and external environment of the infected group’s field (village), pollutants or suspected pollutants shall be treated harmlessly, and the polluted places and facilities shall be thoroughly disinfected. If the infected group is in the trading market or slaughterhouse, the trading market or slaughterhouse should be closed immediately. The trading market or slaughterhouse can only be opened after the provincial veterinary authorities and relevant departments have passed the analysis and evaluation.
Animals other than the infected group in the same field (village) shall be monitored again after the infected group is disposed of, and shall not be allowed to move until there is no positive infection.
Fourth, emergency epidemiological investigation
rightH7If subtype avian influenza virus infection is positive, emergency epidemiological investigation and etiological study shall be conducted with reference to the "Specification for Epidemiological Investigation of Highly Pathogenic Avian Influenza".
V. Personal protection
In emergency response, personnel protection shall be strictly implemented in accordance with the Technical Specification for Personnel Protection of Highly Pathogenic Avian Influenza.